SIGMA| 人血清白蛋白

别名: 
人血清白蛋白*五组分，HSA,

A9511-100MG
A9511-500MG
A9511-1G
A9511-5G
A9511-10G
A9511-25G

研究领域: 
细胞生物学

详述
ALBUMIN, HUMAN

 

The source material for all human albumins made by Sigma has tested negative for HIV and HBSAG.

Sigma tests the solubility of human albumin powders in water at 50 mg/. In the experience of Sigma production chemists, aqueous aliquots stored at –20 °C are stable for several months. Repeated freezing and thawing of solutions is not recommended.

A9511	Albumin from human serum, lyophilized powder, ≥97% (agarose gel electrophoresis),	H2O: soluble50 mg/mL	≥97% (agarose gel electrophoresis)	Sigma	
 
A1653	Albumin from human serum, lyophilized powder, ≥96% (agarose gel electrophoresis),	H2O: soluble50 mg/mL	≥96% (agarose gel electrophoresis)	Sigma	
 
A3782	Albumin from human serum, lyophilized powder, Fatty acid free, Globulin free, ≥99% (agarose gel electrophoresis),	H2O: soluble50 mg/mL	≥99% (agarose gel electrophoresis)	Sigma	
 
A8763	Albumin from human serum, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis),	H2O: soluble20 mg/mL	≥99% (agarose gel electrophoresis)	Sigma	
 
A1887	Albumin from human serum, lyophilized powder, essentially fatty acid free,	H2O: soluble50 mg/mL, clear to slightly hazy, yellow	 	Sigma	
 
A4327	Albumin from human serum, lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis),	H2O: soluble50 mg/mL	≥96% (agarose gel electrophoresis)	Sigma	
 
A5843	Albumin from human serum, lyophilized powder, low endotoxin,	H2O: soluble50 mg/mL	 	Sigma	
 
05420	Albumin from human serum, lyophilized powder, ≥95.0% (agarose gel electrophoresis),	 	≥95.0% (agarose gel electrophoresis)	Sigma
 
 

PRODUCT NO.

 

PREPARATION/PURIFICATION

 

SPECIAL CHARACTERISTICS

 

A 1653

Fraction V1

From human serum.

 

Powder

96-99% by agarose gel (remainder mostly globulins)

 

A 2817

Fraction V1

 

Powder

96-99% by agarose gel (remainder mostly globulins)

Produced specifically for bulk usage.

 

A 1887

Fraction V1, heat step2 Prepared from A 1653.

 

Powder

396% by agarose gel

Essentially fatty acid free (～0.005%)

 

A 9511

 

1× crystallized and lyophilized.

Prepared from A 1653 using method IV of Cohn, et al.3

 

Powder

97-99% by agarose gel

 

A 8763

Prepared from A 1653.4

 

Powder

Essentially globulin free

 

A 3782

Prepared from A 8763.4

 

Powder

Essentially fatty acid free (～0.005%) Essentially globulin free

 

A 4327

 

Prepared from A 1653.

 

Powder

396% by agarose gel

Essentially protease free (<0.0001 u/mg protein) Essentially alkaline phosphatase free (0.001 u/mg protein) Essentially peroxidase free (<0.001 u/mg protein)

 

A 3173

 

Prepared from A 2817.

 

Powder

Low endotoxin content (20.1 ng/mg protein)

 

A 6784

 

Contains approx. 0.85% NaCl. Contains 0.05% NaN3 as preservative. Aseptically filled.

 

10% Solution

 

A 6909

 

Contains approx. 0.85% NaCl. Contains 0.05% NaN3 as preservative. Aseptically filled.

 

30% Solution

 

A 9080

 

From human plasma. Contains approx. 0.85% NaCl. Aseptically filled.

 

30% Solution Protease free.

 

A 2830

 

From human serum.

 

Solution in 40 mM potassium phosphate, pH 7.0

14C-Methylated

5-50 ìCi per mg protein

CAS NUMBER: 70024-90-7

MOLECULAR WEIGHT: 66,248.35 or 66,4378 (based on amino acid composition)

 

STRUCTURE:5

 

The amino acid sequence and structure of human albumin have been determined. Human albumin is a protein with no carbohydrate content. It is a single polypeptide chain with one free sulfhydryl group on residue # 34 and 17 intrachain disulfide bonds.

 

Amino Acid      Asp Asn Thr Ser Glu Gln Pro Gly Ala Cys Val Met Ile Leu Tyr Phe His Lys Trp Arg

Residues         39    15   30   22  60   23  25

 

PHYSICAL  PROPERTIES:5

12 63

35  39   6   8  61 18     30  16  58  1              23

Sedimentation constant, S20,W  X  1013

 

4.6 (monomer), 6.5 (dimer)

Diffusion constant, D20,W  X 107

 

6.1

Partial specific volume, V20

 

0.733

Intrinsic viscosity, ?

 

0.042

Frictional ratio, f/f0

 

1.28

Overall dimensions, ?

 

38 X 150

Isoelectric point (?/2 = 0.15)

 

4.7

Isoionic point (?/2 = 0)

 

5.2

Electrophoretic mobility, pH 8.6, ?/2 = 0.15

 

-5.9

Refractive index increment (578 nm) X   10-3

 

1.89

Optical absorbance, A279 nm  (1  gram/liter)

 

0.531

Mean residue rotation,  [m']233

8590

 

Mean residue ellipticity

 

17 [è]209 nm; 16 [è]222 nm

Estimated á-helix, %

 

48

Estimated a-form, %

 

15

SOLUBILITY / SOLUTION STABILITY:

 

 

 

 


储存与*
储存: 
2-8℃